Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Intraocular pressure and glaucoma pathology in DBA/2J mice (A) Peak IOP measurements from individual eyes in the current study show significantly increased intraocular pressure in D2 mice compared to D2-controls [∗∗∗∗p << 0.0001 with Mann Whitney test; n = 71 mice, 142 eyes (D2-control) and n = 66 mice, 132 eyes (D2)]. Individual data points represent the measurement from an individual eye. (B) Cross section images of optic nerves from 12-month-old D2 and D2-control animals. Scale bars represent 200 μm. (C) Glial scarring as a percentage of cross-sectional optic nerve area was quantified in D2 and D2-controls (∗∗ p = 0.0071 with t test; N = 12 nerves from seven mice [D2-control] and N = 9 nerves from six mice [D2]). Bars and error show mean ± SEM. (D) Individual (gray and light red) and average (black, dark red) pattern electroretinogram (pERG) waveforms from D2-control (black) and D2 (red) mice. n = 16 eyes from eight mice (D2-control) and n = 14 eyes from seven mice. (E) P1 and N2 pERG amplitudes measured from pre-stimulus baseline in D2-control (black) and D2 (red) mice. P1 and N2 amplitude measurements were significantly decreased in D2 mice compared to D2-controls (∗∗∗∗ p = 0.000056 [P1] and ∗∗∗ p = 0.00033 [N2]; n = 16 eyes from eight mice [D2-control] and n = 14 eyes from seven mice [D2]). Bars and error show mean ± SEM. (F) Linear regression of P1 pERG amplitude with maximum IOP, showing a significant correlation. Best fit line and 95% confidence intervals are shown. (G) Linear regression of P1 pERG amplitude with glial scarring, showing a weak but significant correlation. Best fit line and 95% confidence intervals are shown.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: MANN-WHITNEY, Control

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Pharmacology of inhibition indicates decreased activation of extrasynaptic GABA A receptors in DBA/2J TC neurons (A) Example peak-normalized IPSCs from TC neurons from D2 and D2-control mice evoked by stimulation with an aCSF-filled patch pipette positioned approximately 25 microns from the recorded TC neuron in the presence of CNQX (20 μM) and D-AP5 (50 μM). Traces show before (gray) and after (black) bath application of 10 μM DS2. (B) Relative effect of DS2 application on peak response of the IPSC (n.s., p = 0.16 via nested t test) and area (∗∗∗ p = 0.0010 via nested t test) of D2 (red) and D2-control (black) mice ( n = 8 cells, four mice [D2-control] and n = 16 cells, six mice [D2]). Bar graphs and error bars show mean ± SEM. (C) Example peak-normalized traces of IPSCs from D2 and D2-control mice before (gray) and after (black) application of SNAP5114 (60 μM). IPSCs were evoked as in A. (D) Relative effect of SNAP5114 application on peak response (n.s., p = 0.70 via nested t test) and area (n.s., p = 0.91 via nested t test) of D2 (red) and D2-control (black) mice ( n = 16 cells, six mice [D2] and n = 14 cells, five mice [D2-control]). Bar graphs and error bars show mean ± SEM.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: Inhibition, Activation Assay, Control, Transferring

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Intraocular pressure and glaucoma pathology in DBA/2J mice (A) Peak IOP measurements from individual eyes in the current study show significantly increased intraocular pressure in D2 mice compared to D2-controls [∗∗∗∗p << 0.0001 with Mann Whitney test; n = 71 mice, 142 eyes (D2-control) and n = 66 mice, 132 eyes (D2)]. Individual data points represent the measurement from an individual eye. (B) Cross section images of optic nerves from 12-month-old D2 and D2-control animals. Scale bars represent 200 μm. (C) Glial scarring as a percentage of cross-sectional optic nerve area was quantified in D2 and D2-controls (∗∗ p = 0.0071 with t test; N = 12 nerves from seven mice [D2-control] and N = 9 nerves from six mice [D2]). Bars and error show mean ± SEM. (D) Individual (gray and light red) and average (black, dark red) pattern electroretinogram (pERG) waveforms from D2-control (black) and D2 (red) mice. n = 16 eyes from eight mice (D2-control) and n = 14 eyes from seven mice. (E) P1 and N2 pERG amplitudes measured from pre-stimulus baseline in D2-control (black) and D2 (red) mice. P1 and N2 amplitude measurements were significantly decreased in D2 mice compared to D2-controls (∗∗∗∗ p = 0.000056 [P1] and ∗∗∗ p = 0.00033 [N2]; n = 16 eyes from eight mice [D2-control] and n = 14 eyes from seven mice [D2]). Bars and error show mean ± SEM. (F) Linear regression of P1 pERG amplitude with maximum IOP, showing a significant correlation. Best fit line and 95% confidence intervals are shown. (G) Linear regression of P1 pERG amplitude with glial scarring, showing a weak but significant correlation. Best fit line and 95% confidence intervals are shown.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: MANN-WHITNEY, Control

Journal: iScience

Article Title: Compensatory responses to glaucoma pathology in the dorsolateral geniculate nucleus

doi: 10.1016/j.isci.2026.115176

Figure Lengend Snippet: Pharmacology of inhibition indicates decreased activation of extrasynaptic GABA A receptors in DBA/2J TC neurons (A) Example peak-normalized IPSCs from TC neurons from D2 and D2-control mice evoked by stimulation with an aCSF-filled patch pipette positioned approximately 25 microns from the recorded TC neuron in the presence of CNQX (20 μM) and D-AP5 (50 μM). Traces show before (gray) and after (black) bath application of 10 μM DS2. (B) Relative effect of DS2 application on peak response of the IPSC (n.s., p = 0.16 via nested t test) and area (∗∗∗ p = 0.0010 via nested t test) of D2 (red) and D2-control (black) mice ( n = 8 cells, four mice [D2-control] and n = 16 cells, six mice [D2]). Bar graphs and error bars show mean ± SEM. (C) Example peak-normalized traces of IPSCs from D2 and D2-control mice before (gray) and after (black) application of SNAP5114 (60 μM). IPSCs were evoked as in A. (D) Relative effect of SNAP5114 application on peak response (n.s., p = 0.70 via nested t test) and area (n.s., p = 0.91 via nested t test) of D2 (red) and D2-control (black) mice ( n = 16 cells, six mice [D2] and n = 14 cells, five mice [D2-control]). Bar graphs and error bars show mean ± SEM.

Article Snippet: DBA/2J mice (D2, The Jackson Laboratory #000671, RRID:IMSR_JAX:000671) and DBA/2J- Gpnmb1 + mice (D2-control, The Jackson Laboratory #007048, RRID:IMSR_JAX:007048) were used for this study at 11-15 months of age, although most mice in this study were 11-13 months of age.

Techniques: Inhibition, Activation Assay, Control, Transferring

Journal: PLOS One

Article Title: Internal gene segments from a mouse-adapted influenza B virus confer increased pathogenicity to mice

doi: 10.1371/journal.pone.0335324

Figure Lengend Snippet: Different amounts of the 2:6 B/HwNw-Mem97 reassortant with N221S substitution in the C-terminus of BM1 were used to inoculate groups of four BALB/c (a) or DBA/2J mice (b) . Data points represent the mean relative body weight and error bars the standard error of the mean. The experiment was performed using 4 mice per group.

Article Snippet: Specific pathogen-free female BALB/c and DBA/2J mice were obtained from Janvier (France).

Techniques:

Journal: PLOS One

Article Title: Internal gene segments from a mouse-adapted influenza B virus confer increased pathogenicity to mice

doi: 10.1371/journal.pone.0335324

Figure Lengend Snippet: BALB/c ( a ) and DBA/2J mice ( b ) were inoculated with different doses of B/HwNw-Mem97 m.a. virus (obtained after thirteen serial passages in DBA/2J mice). The experiment was performed using 4 mice per group and the LD 50 was calculated using the Reed and Muench method.

Article Snippet: Specific pathogen-free female BALB/c and DBA/2J mice were obtained from Janvier (France).

Techniques: Virus

Journal: bioRxiv

Article Title: Positive allosteric modulator of SERCA pump NDC-1171 attenuates cardiac functional decline in mouse model of Duchenne muscular dystrophy

doi: 10.64898/2026.03.05.709950

Figure Lengend Snippet: Study design outlining treatment of DBA/2J (control), D2. mdx vehicle, and D2. mdx NDC□1171 groups (n = 9 per group). Mice received oral gavage from 6 to 14 weeks of age. Ultrasound transducer icons denote timepoints at which transthoracic echocardiography wa performed (baseline: 6, midpoint: 11, endpoint: 14, and post-behavior: 16 weeks of age; n□=□5–6 per group). Behavioral testing icon represents the functional behavior testing (treadmill exhaustion and four□limb grip strength) conducted at week 15. Mice were euthanized at week 17 and cardiac tissue was collected for histological evaluation.

Article Snippet: Male D2. mdx mice (n=18, strain #013141, D2.B10-Dmdmdx/J) and its background strain DBA/2J mice (Control) were obtained from Jackson Laboratory at 4 weeks of age (n=9).

Techniques: Control, Functional Assay

Journal: bioRxiv

Article Title: Positive allosteric modulator of SERCA pump NDC-1171 attenuates cardiac functional decline in mouse model of Duchenne muscular dystrophy

doi: 10.64898/2026.03.05.709950

Figure Lengend Snippet: D2. mdx vehicle-treated mice (Vehicle) had impairments when compared to DBA/2J vehicle-treated (Control) in both (A) Four-limb grip strength test and (B) Treadmill exhaustion test. D2. mdx mice treated with NDC-1171 did not improve in either behavior assessments. * p <0.05, ** p <0.01, and *** p <0.001.

Article Snippet: Male D2. mdx mice (n=18, strain #013141, D2.B10-Dmdmdx/J) and its background strain DBA/2J mice (Control) were obtained from Jackson Laboratory at 4 weeks of age (n=9).

Techniques: Control